This work investigated the metabolites and their biosynthetic functional hydroxylase genes of the deep-sea sediment metagenomic clone 25D7. 5-Bromoindole was added to the 25D7 clone derived Escherichia coli fermentation broth. The new-generated metabolites and their biosynthetic byproducts were located through LC-MS. in which the isotope peaks of brominated products emerged. https://www.spidertattooz.com/The-GelBottle-Inc-Gel-Polish-Audrey-67oz-p17921/